Journal: bioRxiv
Article Title: Dual targeting of astrocytic and endothelial GLUT1 enables functional rescue in GLUT1 deficiency syndrome
doi: 10.64898/2026.03.04.709430
Figure Lengend Snippet: (A–C) Behavioral analyses in six groups: wild-type, systemic Glut1 del/+ mice, Aldh1l1-Cre (Al cre); Glut1 +/+ mice, Al cre; Glut1 flox/+ mice, Tie2-Cre; Glut1 +/+ mice, and Tie2-Cre; Glut1 flox/+ mice (n = 6–15 mice per group). (A) Object location test (OLT) and (B) novel object recognition test (NORT) were used to assess spatial and recognition memory (discrimination index). (C) The foot-slip test evaluated motor coordination (missed steps per 50 steps). (D) CSF-to-blood glucose ratio measured in the same cohorts (n = 5–6 mice per group). Blood glucose levels were comparable across groups (see ). (E) Schematic of the in vivo recording setup for thalamic interstitial fluid (ISF) glucose measurement. Illustration created with BioRender. (F) ISF glucose measured following oral glucose administration (0.03 g per mouse, gavage). The increase in ISF glucose was markedly blunted in systemic, astrocyte-specific, and endothelial-specific Glut1 haploinsufficient mice compared with wild-type controls (n = 4–5 mice per group). Data are presented as mean ± SEM; dots represent individual mice. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple-comparison test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
Article Snippet: Mice harboring the Tie2-Cre (stock no. #004128), Aldh1l1-Cre (stock no. #023748), Aldh1l1-CreERT2 (stock no. #029655) drivers, tdTomato-floxed STOP reporter mice (tdTomato reporter mice) (stock no. 007914) and floxed Glut1 mice (stock no. #031871) were all obtained from the Jackson Laboratory, backcrossed over 10 generations to the C57BL/6J genetic strain background.
Techniques: In Vivo, Comparison
